CRISPR-Cas: A Laboratory Manual by Jennifer Doudna
- CRISPR-Cas: A Laboratory Manual
- Jennifer Doudna
- Page: 450
- Format: pdf, ePub, mobi, fb2
- ISBN: 9781621821311
- Publisher: Cold Spring Harbor Laboratory Press
Ebooks free download deutsch pdf CRISPR-Cas: A Laboratory Manual ePub
CRISPR-Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for designing guide RNAs that precisely target the gene of interest, delivering CRISPR-Cas reagents into cells (e.g., using AAV vectors), and selecting cells that harbor the desired changes. Strategies for scaling and multiplexing these procedures are also described, as are methods for optimizing CRISPR-Cas systems in common species and cell lines, from yeast to human.
The CRISPR/Cas Immune System Is an Operon Regulated by LeuO
The results obtained show that in S. Typhi the CRISPR/Cas system is a LeuO- dependent operon silenced Molecular cloning: a laboratory manual, 2nd ed.
Guide-it™ CRISPR/Cas9 Systems User Manual - Clontech
Clontech Laboratories, Inc. Guide-it™. CRISPR/Cas9 Systems. User Manual. Cat . No(s). 632601, 632602. (033015). Clontech Laboratories, Inc. A Takara Bio
Cold Spring Harbor Lab Press Cell Biology
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego.
Patent US8697359 - CRISPR-Cas systems and methods - Google
The CRISPR-Cas system of claim 15 , wherein the expression of two or and Maniatis, MOLECULAR CLONING: A LABORATORY MANUAL,
Crystal structure of a crispr-cas system, and uses thereof - Google
Also provided are methods of directing CRISPR complex formation in eukaryotic cells 2, "ANTIBODIES, A LABORATORY MANUAL", 1988.
2 - Cold Spring Harbor Lab Press
Budding Yeast: A Laboratory Manual Edited By Brenda Andrews, University of this Product · CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna,
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